Identification of Primordial Germ Cells: Cytological, Histological and Immunohistochemical Aspects

Review of Differentiation and Proliferation of Primordial Germ Cells in Culture

Primordial germ cells (PGCs) are highly specialized cell population that arises from the epiblast in vivo. There are three critical steps in the life cycle of these cells: 1-Specification 2-migration and proliferation 3-prenatal and postnatal sex specific development. Specification of germ cells in epiblast occurs due to signals secreted from extraembryonic tissues. Primordial germ cells are re...

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

The effect of BMP4 on mouse embryonic stem cell proliferation and differentiation into primordial germ cells

Background and Aim: Artificial gamete production from stem cells is a novel strategy for treatment of infertility. Among various stem cell sources, embryonic stem cells (ESC) can be considered as an appropriate source for in vitro formation of germ cells. In this study we evaluated the effect of BMP4 on proliferation and differentiation of mouse embryonic stem cells into primordial germ cells (...

Early sex differentiation in Mugil ( Liza ) auratus Risso , 1810 ( Teleost Mugilidae ) . An ultrastructural study

Grey-mullet are heterosexual fish and gonochorism is the general rule. Cytological cnalysis of sex-differentiation was studied by light microscopy (Stenger, 1959 ; Leray, 1968 ; Thong, 1969 ; Cassifour, 1975), but germ-cell characteristics were not sufficiently defined at this level and no distinction could be made between primordial germ-cells, oogonia and spermatogonia. Electron microscopy ha...

The Role of Wnt/β-catenin Signaling Pathway in Rat Primordial Germ Cells Reprogramming and Induction into Pluripotent State

 Primordial Germ Cells (PGCs) are unipotent precursors of the gametes. PGCs can give rise to a type of pluripotent stem cells in vitro that are called embryonic germ (EG) cells. PGCs can also acquire such pluripotency in vivo and generate teratomas. Under specific culture conditions, PGCs can be reprogrammed to embryonic germ cells which are capable of expression of key pluripotency marker...